Biochemical Techniques
Android app by StudyZoom. Education · StudyZoom
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- Unknown
- Store rating count
- Unknown
- Download price
- Free to download
- In-app purchases
- Listed by the store
- Version
- 1.2
- Listing last refreshed
- 2026-09-11
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Store description excerpt
📘 Biochemical Techniques (2026–2027 Edition) Biochemical Techniques (2026–2027 Edition) is a research-oriented textbook for Biochemistry, Biotechnology, Life Sciences, Molecular Biology, & Bioanalytical Science students. It covers lab fundamentals, centrifugation, electrophoresis, chromatography, spectroscopy, immunochemical methods, molecular biology, protein purification, radiochemical techniques, & structural instrumentation. Full syllabus with unit-wise chapters, topic-wise coverage, MCQs, & quizzes for exams, lab research & higher study. 📗 Unit 1: Laboratory Basics, Safety & Sample Handling • Lab organisation & SOPs • Biosafety, hazard classification, waste disposal • Aseptic technique & contamination control • Pipette prep, calibration & validation • Buffer systems, pH measurement, titration curves 📘 Unit 2: Centrifugation & Subcellular Fractionation • Centrifugal force principles & rotor types • Differential centrifugation (nuclei, mitochondria) • Density gradient separation (sucrose, CsCl, Percoll) • Sedimentation coefficients & purification metrics • Organelle fractionation protocols 📙 Unit 3: Electrophoretic Separation Techniques • Electrophoresis principles: charge, size, matrix • Agarose gel electrophoresis of nucleic acids • Native PAGE vs SDS-PAGE • 2D electrophoresis (IEF + SDS-PAGE) • Isoelectric focusing & pH gradient gels 📕 Unit 4: Chromatography Methods • Adsorption vs partition theory • Paper chromatography & Rf interpretation • Thin-layer chromatography (TLC) prep & detection • Gel-filtration (size-exclusion) for proteins • Ion-exchange chromatography (cation/anion) 📒 Unit 5: Spectroscopy & Spectrometry • Beer–Lambert law & calibration • UV-Vis spectrophotometry (DNA, proteins) • Fluorescence spectroscopy principles • Flame photometry & atomic absorption • Mass spectrometry (MALDI-TOF, LC-MS, GC-MS) 📔 Unit 6: Immunochemical & Binding Techniques • Antigen–antibody binding & specificity • ELISA: direct, indirect, sandwich, competitive • Western blotting: transfer, blocking, detection • Immunofluorescence & immunohistochemistry • Co-immunoprecipitation & pull-down assays • Monoclonal vs polyclonal antibody prep 📘 Unit 7: Molecular Biology Techniques • Genomic DNA & total RNA isolation • Restriction digestion, ligation, cloning, plasmids • PCR: conventional, multiplex, qPCR • RT-PCR & real-time gene expression • Southern, Northern, Western blotting • DNA sequencing: Sanger & NGS overview 📗 Unit 8: Protein Purification & Activity Analysis • Protein assays: Biuret, Lowry, Bradford, BCA • Fractionation: crude to purified protein • Chromatography: affinity, ion-exchange, size • Dialysis, ultrafiltration, buffer exchange • Molecular weight via SDS-PAGE & gel filtration • Enzyme activity assays & kinetics 📘 Unit 9: Radiochemical & Tracer Techniques • Radioisotope basics: half-life, decay, units • α, β, γ radia
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